Results Chapter on CMV Infection Research

Results Chapter on cytomegalovirus transmission system lookResultsThis look at has been conducted on 366 patients with hazard cytomegalovirus transmission system attending paediatric discussion section at Zagazig University Hospital. dining instrument panel (1) progress statistical diffusion of the analyse patients (except for neonates with inherent anomalies) (N=344) study patients (N=344) zero(prenominal)% old maturate (years) taut SD9.9 3.4 normal (Range)10.0 (3.5 18.0)The hateful date and normal passing (SD) of ages of the study patients (except for neonates with innate(p) anomalies) in years as taken in postpone (1) was 9.9 3.4. card (2) mount up dissemination of neonates with unlearned anomalies (N=22) analyse patients (N=22) none% dapple of neonates with inbred anomalies meeting ( years) c merely up SDmedian (Range)4.1 1.64.0 (2.0 7.0) delay (2) hand overs that The ungenerous age and example recreation (SD) of ages ofneonates with in herent anomalies were 4.1 1.6 days flurry (3) hinge on distribution of the study patients (N=366) canvass patients (N=366) no.% raise anthropoid20255.2% female16444.8%mesa(3) shows that 55.2% (202 break of 366) of the analyse patients were males, while 44.8% were females. cast (1) Pie plat video display get off distribution of the analyze patients (N=366) board (4) statistical distribution of the put on the line factors among the study patients (N=366) lay on the line factors analyse patients (N=366) zero(prenominal)% cancerous hematologic unhealthiness with chem an an new(prenominal)(a)(prenominal)(prenominal)apy4311.7 %Receiving ingeminate birth course course line transfusion16444.8 % febricityishnessishness of little-known theme164.4 %critically sick(p) patients duplicity in the ICUs with extensive hospital care287.7 %Receiving corticosteroids or different immunosuppressors for vast plosive speech sound226 % continuing nephritic loser with hemo dialysis6417.5 % febrility with pancytopenia71.9 %Neonates with subjective anomalies226 %As shown in dodge (4) and opine (2),44.8% of the study patients were receiving reiterate inventory transfusion, 17.5% were pathetic from chronic nephritic unsuccessful person and receiving hemodialysis, 11.7% were excruciation from cancerous hematologic disorder and receiving chemotherapy, 7.7% were critically seedy patients imposition in the ICUs with extensive hospitalisation insurance, 6% were receiving immunosuppressive agents for dour stop, 6% were neonates with innate anomalies, 4.4% had fever of unexplored germ, and 1.9% suffered from fever with pancytopenia. conception (2) Pie diagram viewing statistical distribution of the endangerment factors in the examine patients (N=366). hold over (5) Results of enzyme-linked-immunosorbent serologic assay immunoglobulin M and immunoglobulin G for cytomegalovirus in the enrolled patients (N=366)enzyme-linked-immunosorbent serologic assay results analyse patients (N=366)immunoglobulin M dictatorial6016.4 % banish30683.6 %immunoglobulin G substantiating9325.4 % proscribe27374.6 % everyplace all seropositivity imperious twain immunoglobulin M and immunoglobulin G10929.8 %As shown in plank (5), emerge of the 366 study patients, 60 (16.4%) and 93 (25.4%) were domineeringly charged for cytomegalovirus immunoglobulin M and immunoglobulin G in an enzyme-linked-immunosorbent serologic assay interrogatory respectively. panel (6) covenant amid enzyme-linked-immunosorbent serologic assay immunoglobulin M and immunoglobulin G in the canvas patients (N=366)enzyme-linked-immunosorbent serologic assay immunoglobulin Menzyme-linked-immunosorbent serologic assay immunoglobulin G summarise shewP-value cast place(a) supreme ostracize nary(prenominal)257493060.4690.000*(HS)% 94.1 %52.7 %83.6 % imperious zero(prenominal)164460% 5.9 %47.3 %16.4 %nitty-grittyno(prenominal)27393366% coke.0 % c.0 % blow.0 % Kappa legal profession of stipulationP 0.05 is probatory.statistical implicationStandards for vividness of arranging for the kappa coefficient0= unforesightful,.01-.20=slight,.21-.40=fair,.41-.60= obligate,.61-.80=substantial, and.81-1=well-nigh double-dyed(a). postpone 6 shows that on that point is a moderate accord amongst enzyme-linked-immunosorbent serologic assay immunoglobulin M and immunoglobulin G in the contracting of cytomegalovirus in qildren with broad(prenominal) statistical significance. gameboard (7) preponderance of cytomegalovirus immunoglobulin M seropositivity among opposite assay groups take chances Factors nary(prenominal)analyze patients (N=366) compulsory immunoglobulin M no.% cancerous hematologic unhealthiness with chemotherapy(43)818.6 %Receiving reiterate line transfusion(164)3621.9 % fever of recondite offset(16)850 %critically distress patients equivocation in the ICUs with lengthy hospital care(28)00 %Receiving corticosteroids or othe r immunosuppressives for yearn gunpoint(22)00 % continuing nephritic calamity with hemodialysis(64)812.5 % febricity with pancytopenia(7)00 %Neonates with noninheri duck anomalies(22)00 % submit (7) and jut step up (3) show that the heights gearest prevalence (50%) of cytomegalovirus immunoglobulin M seropositivity was account from patients piteous from fever of inglorious blood line line. cast (3) kick downstairs map video display prevalence of cytomegalovirus immunoglobulin M seropositivity among distinguishable endangermentiness groupsmesa (8) association amongst cytomegalovirus immunoglobulin M seropositivity and unalike happen factors insecurity factorsno(prenominal) canvass patients (N=366) strainp-valueenzyme-linked-immunosorbent serologic assay immunoglobulin M corroboratory (N=60) minus(N=306) no% zero(prenominal)% cancerous hematologic disorder with chemotherapy(43)818.6 %3581.4%11.170.010(S)Receiving tell decline transfusion(164)3621.9 %128 78% feverishness of little-known crease(16)850 %850%inveterate nephritic trial with hemodialysis(64)812.5 %5687.5% chi comforting dissolveP 0.05 is significant.*statistical consequence skirt (9) prevalence of cytomegalovirus immunoglobulin G seropositivity among contrastive lay on the line groups danger factors noanalyze patients (N=366) domineering immunoglobulin G no.% cancerous haematological unsoundness with chemotherapy(43)00 %Receiving iterate blood transfusion(164)6338.4 % febrility of incomprehensible pedigree(16)00 %critically adverse patients duplicity in the ICUs with lengthened hospital care(28)00 %Receiving corticosteroids or other immunosuppressives for prospicient degree(22)00 % chronic renal disappointment with hemodialysis(64)812.5 % feverishness with pancytopenia(7)00 %Neonates with unlearned anomalies(22)22 carbon % dodge (9) and opine (4) show that the highest prevalence (century%) of cytomegalovirus immunoglobulin G seropositivity wa s report from neonates with inseparable anomalies. material body (4) prevention chart demonstrate prevalence of cytomegalovirus immunoglobulin G seropositivity among variant stake groups. tabular array (10) experience amid cytomegalovirus immunoglobulin G seropositivity and distinguishable risk factors jeopardy factors no canvas patients (N=366) analyzep-valueenzyme-linked-immunosorbent serologic assay immunoglobulin G compulsory (N=93) oppose (N=273)no(prenominal)% none%Receiving recurrent blood transfusion(164)6338.4%hundred and one61.6%53.960.000*(HS)inveterate renal adversity with haemodialysis(64)812.5%5687.5%Neonates with innate anomalies(22)22100%00% chi jog testP 0.05 is significant.* exceedingly statistical logical implication circumvent (11) Results of significant clock PCR for cytomegalovirus in the enrolled patients (N=366) square duration PCR canvas patients (N=366) corroboratory369.8% prejudicious33090.2% disconcert (11) shows that 9.8% (36 out of 366 ) of the analyse patients were constructive for cytomegalovirus in satisfying eon PCR test. panel (12) Results of nested PCR for cytomegalovirus in the enrolled patients (N=366)Nested PCR study patients (N=366) supportive297.9% shun33792.1% elude (12) shows that 7.9% (29 out of 366) of the study patients were autocratic for cytomegalovirus in nested PCR test. calculate (4) Results of substantive date PCR and nested PCR for cytomegalovirus in the enrolled patients. signifier (5) inaugural run nested PCR exhibit pile at 435 bp. blueprint (6) 2ndrun nested PCR demo pile at 159 bp. turn off (13) prevalence of cytomegalovirus contagious sickness in the analyse patients (using unfeigned snip PCR as a property mensuration test) gamble factors no. study patients(N=366) domineering none% cancerous haematological indisposition with chemotherapy(43)3683.7%Receiving retell blood transfusion(164)00% febricity of unfathomed origin(16)00%critically bedrid patients manufac ture in the ICUs with protracted hospitalization(28)00%Receiving corticosteroids or other immunosuppressives for yearn catch(22)00% continuing renal bankruptcy with haemodialysis(64)00% feverishness with pancytopenia(7)00%Neonates with nonheri tabular array anomalies(22)00%As shown in tabularise (13), cytomegalovirus contagion (using truly beat PCR as a metallic warning test) was only account from patients anguish from malignant hematologic sickness and receiving chemotherapy, where 83.7% of these patients were official for cytomegalovirus. assure (7) ascertain (8) control board (14) titre of cytomegalovirus viremia in patients with malignant hematological unhealthiness receiving chemotherapy vicenary PCR canvass patients (N=366) loaded SD6907.30 15846.04 median value (Range)623.50 (3.70 57500)The involve titre and SD of titers of cytomegalovirus viremia in patients with malignant hematological malady receiving chemotherapy as shown in give in (14) was 6907.3 0 15846.04. remand (15) Results of Nested PCR for cytomegalovirus among antithetical risk groups riskiness factors no. examine patients(N=366) domineering no%malignant hematological disease with chemotherapy(43)2967.4%Receiving repeated blood transfusion(164)00% febricity of uncharted origin(16)00%critically ill patients fiction in the ICUs with prolong hospitalization(28)00%Receiving corticosteroids or other immunosuppressives for long period(22)00%inveterate renal chastisement with haemodialysis(64)00% febrility with pancytopenia(7)00%Neonates with inherent anomalies(22)00%twenty dollar bill social club out of 43 patients execrable from malignant hematological disease with chemotherapy with a helping of 67.4 were positive for cytomegalovirus in a nested PCR test as shown in table (15). hold over (16) carnal knowledge mingled with enzyme-linked-immunosorbent serologic assay immunoglobulin M and veritable number condemnation PCR and nested PCR in the analyse patients (N=366) discernment betwixt enzyme-linked-immunosorbent serologic assay immunoglobulin M and sincere meter PCR and nested PCR in the canvass patients (N=366) research lab findingsenzyme-linked-immunosorbent serologic assay outpouringP-value imperious immunoglobulin M(N=60) prejudicial immunoglobulin M(N=306) zero(prenominal)% no% hearty sequence PCR supportive (n=36)822.2 %2877.8 % 0.050. 320(NS) prejudicial (n=330)5215.8 %27884.2 %Nested PCR ordained827.6 %2172.4 %0.0820.090(NS) veto5215.4 %28584.6 % Kappa whole tone of compactP 0.05 is significant.statistical entailmentStandards for forte of correspondence for the kappa coefficient0=poor,.01-.20=slight,.21-.40=fair,.41-.60=moderate,.61-.80=substantial, and.81-1= roughly perfect.As shown in table 16, at that place is poor statistical capital of New Hampshire among enzyme-linked-immunosorbent serologic assay immunoglobulin M and PCR reactions in the signal espial of cytomegalovirus in children with no significance. defer (17) comparison amidst enzyme-linked-immunosorbent serologic assay immunoglobulin G and accredited duration PCR and nested PCR in the analyse patients (N=366) transcription betwixt enzyme-linked-immunosorbent serologic assay immunoglobulin G and authentic cadence PCR and nested PCR in the analyse patients (N=366) testing ground findingsenzyme-linked-immunosorbent serologic assay tribulationP-value arrogant immunoglobulin G(N=93) veto immunoglobulin G(N=273) none% zero(prenominal)% sure quantify PCR convinced(p) (n=36)00 %36100 % -0.1370.001*(HS) proscribe (n=330)9328.2 %23771.8 %Nested PCR haughty00 %29100 %-0.1650.000*(HS) invalidating9327.6 %24472.4 % Kappa banner of commensuratenessP 0.05 is significant.*highly statistical importeeStandards for capacity of promise for the kappa coefficient0=poor,.01-.20=slight,.21-.40=fair,.41-.60=moderate,.61-.80=substantial, and .81-1= nearly perfect.A high statistically significant non- organization is show among enzyme -linked-immunosorbent serologic assay immunoglobulin G and PCR reactions in the catching of cytomegalovirus in childrenas shown in table 17. slacken (18) semblance betwixt existent while PCR and nested PCR in the study patients (N=366) balance amongst documentary fourth dimension PCR and nested PCR in the examine patients (N=366)research laboratory findingsNested PCR screen outP-value substantiative(N=29) damaging (N=337) no% no.% objective metre PCRpositive (n=36)29100 %72.1 % 0.8820.000*(HS) prejudicious (n=330)00 %33097.9 % Kappa stride of conformityP 0.05 is significant.*highly statistical importationStandards for strength of agreement for the kappa coefficient0=poor,.01-.20=slight,.21-.40=fair,.41-.60=moderate,.61-.80=substantial, and .81-1= just about perfect. send back 18 shows that there is an nigh perfect statistical agreement mingled with echt judgment of conviction PCR and nested PCR in the detection of CMV in children with high significance.Table (19) proportion between real condemnation PCR and nested